| Instrument | Autopurification system | |
| Column | Xbridge prep C18 OBD, 19 mm × 250 mm, 5μm | |
| Detector | UV-VIS detector (254, 365 nm) | |
| Solvent A | Acetonitrile | |
| Solvent B | 0.1% Formic acid in water | |
| Flow rate | 9.0 mL/min | |
| oven | ||
| Injection volume | 100 μL | |
| Gradient elution system | ||
| Time (min) | %A | %B |
| Initial | 65 | 35 |
| 2 | 65 | 35 |
| 10 | 65 | 35 |
| 60 | 100 | 0 |
| 80 | 100 | 0 |
| 81.05 | 65 | 35 |
| 82 | 65 | 35 |