Table 3. Chromatography Settings for BPCA analysis(Wiedemeier et al., 2016).

Column	C18 reversed phase
Column temperature	15°C
Flow rate	0.4 ml/min
Identification	Retention time, UV absorption at 216 nm
Quantification	External stnadards of BPCAs
Pressure	120 bar
Mixing Gradient of Mobile Phases
Time[min]	Mobile phase B[vol%]
0	0.5
5	0.5
25.9	30
26	95
28	95
28.1	0.5
30	0.5