Table 2. Effect of the concentration of cytokinins (BAP, zeatin, kinetin) and auxin (NAA) added to MS on callus induction from leaf segments after 12 weeks of culturez.

Growth regulators (mg/L) No. of leaf segments cultured No. of leaf segments produced callus % callus induction % organogenesis induction Length size of callus(mm)y Fresh weight (mg) of callus/explantx
BAP zeatin kinetin NAA
0.0 0.0 0.0 0.0 15 0 - - 0.0±0.0 0.0±0.0
1.0 1.0 15 12 80 - 14.67±0.95 180.4±40.83
1.0 2.0 15 15 100 6.7 16.67±0.94* 295.8±45.53*
1.0 3.0 15 13 86.7 - 14.36±0.77 149.8±38.08
1.0 1.0 15 10 66.7 26.7 11.32±0.18 182.4±66.14
1.0 2.0 15 15 100 33.3 13.27±0.74 183.73±33.45
1.0 3.0 15 12 80 53.3 14.15±0.30 293.73±60.8
1.0 1.0 15 1 6.7 - 9.08±0.94 18.06±1.2
1.0 2.0 15 2 13.3 - 9.84±0.94 64.93±29.72
1.0 3.0 15 1 6.7 - 9.57±0.98 33.2±14.26
Medium used was MS with 3% sucrose and 0.8% plant agar. Cultures were maintained in light conditions for 12 weeks.
Designate callus length size of mean ± standard error with three replications.
Designate callus fresh weight of mean ± standard error with three replications.
The different letters in column indicate significantly difference at p < 0.05 by one-way ANOVA.