Table 2. Effect of the concentration of cytokinins (BAP, zeatin, kinetin) and auxin (NAA) added to MS on callus induction from leaf segments after 12 weeks of culturez.
| Growth regulators (mg/L) | No. of leaf segments cultured | No. of leaf segments produced callus | % callus induction | % organogenesis induction | Length size of callus(mm)y | Fresh weight (mg) of callus/explantx |
| BAP | zeatin | kinetin | NAA |
| 0.0 | 0.0 | 0.0 | 0.0 | 15 | 0 | - | - | 0.0±0.0 | 0.0±0.0 |
| 1.0 | | | 1.0 | 15 | 12 | 80 | - | 14.67±0.95 | 180.4±40.83 |
| 1.0 | | | 2.0 | 15 | 15 | 100 | 6.7 | 16.67±0.94* | 295.8±45.53* |
| 1.0 | | | 3.0 | 15 | 13 | 86.7 | - | 14.36±0.77 | 149.8±38.08 |
| 1.0 | | 1.0 | 15 | 10 | 66.7 | 26.7 | 11.32±0.18 | 182.4±66.14 |
| 1.0 | | 2.0 | 15 | 15 | 100 | 33.3 | 13.27±0.74 | 183.73±33.45 |
| 1.0 | | 3.0 | 15 | 12 | 80 | 53.3 | 14.15±0.30 | 293.73±60.8 |
| | 1.0 | 1.0 | 15 | 1 | 6.7 | - | 9.08±0.94 | 18.06±1.2 |
| | 1.0 | 2.0 | 15 | 2 | 13.3 | - | 9.84±0.94 | 64.93±29.72 |
| | 1.0 | 3.0 | 15 | 1 | 6.7 | - | 9.57±0.98 | 33.2±14.26 |
Medium used was MS with 3% sucrose and 0.8% plant agar. Cultures were maintained in light conditions for 12 weeks.
Designate callus length size of mean ± standard error with three replications.
Designate callus fresh weight of mean ± standard error with three replications.
The different letters in column indicate significantly difference at p < 0.05 by one-way ANOVA.