Table 3. Effect of cytokinin (BAP) and auxin (NAA) added to MS on callus induction from leaf segments after 12 weeks of culturez.

Growth regulators (mg/L) No. of leaf segments cultured No. of leaf segments produced callus % callus induction Length size of callus(mm)y Fresh weight (mg) of callus/explantx
BAP NAA
0.0 0.0 15 0 - 0.0±0.0 0.0±0.0
0.5 1.0 15 2 13.3 10.37±0.52 34±5.05
0.5 2.0 15 12 80 17.62±1.14* 283.3±52.65
0.5 3.0 15 10 66.7 13.68±0.81 80±11.91
1.0 1.0 15 12 80 14.67±0.95 180.4±40.83
1.0 2.0 15 15 100 16.67±0.94 295.8±45.53*
1.0 3.0 15 13 86.7 14.36±0.77 149.8±38.08
Medium used was MS with 3% sucrose and 0.8% plant agar.
Cultures were maintained in light conditions for 12 weeks.
Designate callus length size of mean ± standard error with three replications.
Designate callus fresh weight of mean ± standard error with three replications.
The different letters in column indicate significantly difference at p < 0.05 by one-way ANOVA.