Table 3. Effect of cytokinin (BAP) and auxin (NAA) added to MS on callus induction from leaf segments after 12 weeks of culturez.
| Growth regulators (mg/L) | No. of leaf segments cultured | No. of leaf segments produced callus | % callus induction | Length size of callus(mm)y | Fresh weight (mg) of callus/explantx |
| BAP | NAA |
| 0.0 | 0.0 | 15 | 0 | - | 0.0±0.0 | 0.0±0.0 |
| 0.5 | 1.0 | 15 | 2 | 13.3 | 10.37±0.52 | 34±5.05 |
| 0.5 | 2.0 | 15 | 12 | 80 | 17.62±1.14* | 283.3±52.65 |
| 0.5 | 3.0 | 15 | 10 | 66.7 | 13.68±0.81 | 80±11.91 |
| 1.0 | 1.0 | 15 | 12 | 80 | 14.67±0.95 | 180.4±40.83 |
| 1.0 | 2.0 | 15 | 15 | 100 | 16.67±0.94 | 295.8±45.53* |
| 1.0 | 3.0 | 15 | 13 | 86.7 | 14.36±0.77 | 149.8±38.08 |
Medium used was MS with 3% sucrose and 0.8% plant agar.
Cultures were maintained in light conditions for 12 weeks.
Designate callus length size of mean ± standard error with three replications.
Designate callus fresh weight of mean ± standard error with three replications.
The different letters in column indicate significantly difference at p < 0.05 by one-way ANOVA.